Microscope Image Size Calculator
Calculate real-world size from microscope images
Convert pixels to micrometers, millimeters, and more
Microscope Settings
Camera Settings
💡 Quick Tips
Pixel Size: The real-world size each pixel represents. Smaller values mean higher resolution.
Field of View: The total area visible in your image. Useful for understanding spatial context.
Magnification: Use the objective lens magnification (4×, 10×, 40×, etc.). Include any additional camera adapters.
Sensor Size: Found in camera specifications. Most scientific cameras use 1/2" or 1/3" sensors.
How to Use
Select Microscope Settings
Choose your microscope brand and magnification, or enter custom values. Common microscopes like Olympus, Nikon, and Zeiss are pre-configured.
Enter Camera Information
Select your camera model or manually input sensor size and image dimensions. Presets available for common scientific cameras.
Calculate Pixel Size
Instantly see the real-world size of each pixel in micrometers. Use this value to measure objects in your microscope images.
Measure Objects
Use the measurement tool to draw lines on your image. Get instant measurements in micrometers, millimeters, or other units.
Supported Microscopes
🔬 Olympus
- • BX Series (BX41, BX43, BX51, BX53)
- • CX Series (CX23, CX33, CX43)
- • Magnifications: 4×, 10×, 20×, 40×, 100×
🔬 Nikon
- • Eclipse Series (Ci, Ni, Ti2)
- • Optiphot Series
- • Magnifications: 4×, 10×, 20×, 40×, 60×, 100×
🔬 Zeiss
- • Axio Series (Axio Lab, Axio Scope)
- • Primo Star
- • Magnifications: 5×, 10×, 20×, 40×, 63×, 100×
Perfect For
🧬 Biological Research
- • Cell size measurement
- • Tissue analysis
- • Bacterial colony sizing
- • Organelle quantification
🎓 Education
- • Biology lab assignments
- • Student research projects
- • Teaching demonstrations
- • Scientific reports
🏥 Medical Research
- • Pathology analysis
- • Histology measurements
- • Blood cell counting
- • Tissue morphometry
⚙️ Material Science
- • Crystal structure analysis
- • Surface morphology
- • Grain size measurement
- • Particle size distribution
Calculation Formula
Pixel Size Formula
Example Calculation
• Camera sensor: 6.4 mm wide
• Image resolution: 1280 pixels wide
• Magnification: 40×
Result: Pixel Size = 6.4 × 1000 / (1280 × 40) = 0.125 μm per pixel
This means each pixel in your image represents 0.125 micrometers in the real world.
Frequently Asked Questions
How do I find my camera sensor size?
Check your camera's manual or specification sheet. For scientific cameras, sensor size is usually listed in millimeters (e.g., 6.4mm × 4.8mm). You can also select from our presets if your camera model is listed.
What magnification should I use?
Use the total magnification of your microscope setup. This is the objective lens magnification (e.g., 40×) multiplied by any additional magnification (like a 2× camera adapter). For most cases, it's just the objective magnification.
Can I use this for scanning electron microscope (SEM) images?
Yes, but SEM images usually have a scale bar embedded. If you know the pixel size from the scale bar, you can use our Area Calculator tool instead. This tool is designed primarily for optical microscopy.
Why is pixel size important?
Knowing the pixel size allows you to convert measurements in pixels to real-world units (micrometers, millimeters). This is essential for scientific analysis, publications, and accurate comparisons between images.
Is this tool accurate?
Yes, the calculations are mathematically precise. However, accuracy depends on correct input values (camera sensor size, magnification). For critical measurements, verify with a calibration slide.
Can I save my microscope settings?
Currently, settings are stored in your browser's local storage. You can bookmark the page and your last used settings will be preserved for future visits.
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